immunocal reagent Search Results


human recombinant macrophage inflammatory protein mip 1a nibsc centralised facility for aids reagents  (R&D Systems)
90
R&D Systems human recombinant macrophage inflammatory protein mip 1a nibsc centralised facility for aids reagents
Human Recombinant Macrophage Inflammatory Protein Mip 1a Nibsc Centralised Facility For Aids Reagents, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/immunocal+reagent/Recombinant+Human+CCL3%2FMIP-1+alpha+Protein%2C+CF/pm11290759-70-1-15
Average 90 stars, based on 1 article reviews
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fitc conjugated anti rabbit  (Jackson Immuno)
94
Jackson Immuno fitc conjugated anti rabbit
Fitc Conjugated Anti Rabbit, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/immunocal+reagent/Fluorescein+(FITC)+AffiniPure+Rabbit+Anti-Horseradish+Peroxidase/pm16791003-88-3-5
Average 94 stars, based on 1 article reviews
fitc conjugated anti rabbit - by Bioz Stars, 2026-09
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mouse anti rat igg fitc  (Jackson Immuno)
93
Jackson Immuno mouse anti rat igg fitc
Mouse Anti Rat Igg Fitc, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/immunocal+reagent/Fluorescein+(FITC)+AffiniPure+Mouse+Anti-Rat+IgG/pm16791882-191-4-18
Average 93 stars, based on 1 article reviews
mouse anti rat igg fitc - by Bioz Stars, 2026-09
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ccr5  (ATCC)
90
ATCC ccr5
Ccr5, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/immunocal+reagent/2D-7/pmc01797449-72-39-52
Average 90 stars, based on 1 article reviews
ccr5 - by Bioz Stars, 2026-09
90/100 stars
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efavirenz  (Selleck Chemicals)
92
Selleck Chemicals efavirenz
Efavirenz, supplied by Selleck Chemicals, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/immunocal+reagent/Efavirenz/bio_rxiv__2024__09__06__611547-192-11-21
Average 92 stars, based on 1 article reviews
efavirenz - by Bioz Stars, 2026-09
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p24  (ATCC)
96
ATCC p24
The effectiveness of Vpx incorporation into anti-HIV-1 gene therapy vectors. Macrophages were transduced with either Cal-1, PromA or their respective controls packaged with or without Vpx. At 14 dpt, the macrophages were infected with R5 BaL at MOI 0.2. After 7 days of infection, the cells were fixed and stained for <t>p17/p24</t> and counterstained with DAPI. The cells were imaged on the Cytell and analysed using IN Cell Investigator software. Using DAPI as the denominator the percentage of transduced cells was calculated for Cal-1 ( a ) and shPromA ( b ), the percentage of infected cells for Cal-1 ( c ) and shPromA ( e ), and the percentage of transduced and infected cells for Cal-1 ( d ) and shPromA ( f ). The nonparametric Friedman test was used for statistical analyses. P ≤ 0.05 (*), P ≤ 0.01 (**) and P ≤ 0.001 (***). Mean + SD were plotted; n = 3. One representative donor of three is displayed. DAPI, 4,6-diamidino-2-2-phenylindole; MOI, multiplicity of infection; SD, standard deviation.
P24, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/immunocal+reagent/Aspergillus+niger+van+Tieghem/pmc05070512-211-12-8
Average 96 stars, based on 1 article reviews
p24 - by Bioz Stars, 2026-09
96/100 stars
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pnl4 3 deltaenv gfp  (ATCC)
94
ATCC pnl4 3 deltaenv gfp
The effectiveness of Vpx incorporation into anti-HIV-1 gene therapy vectors. Macrophages were transduced with either Cal-1, PromA or their respective controls packaged with or without Vpx. At 14 dpt, the macrophages were infected with R5 BaL at MOI 0.2. After 7 days of infection, the cells were fixed and stained for <t>p17/p24</t> and counterstained with DAPI. The cells were imaged on the Cytell and analysed using IN Cell Investigator software. Using DAPI as the denominator the percentage of transduced cells was calculated for Cal-1 ( a ) and shPromA ( b ), the percentage of infected cells for Cal-1 ( c ) and shPromA ( e ), and the percentage of transduced and infected cells for Cal-1 ( d ) and shPromA ( f ). The nonparametric Friedman test was used for statistical analyses. P ≤ 0.05 (*), P ≤ 0.01 (**) and P ≤ 0.001 (***). Mean + SD were plotted; n = 3. One representative donor of three is displayed. DAPI, 4,6-diamidino-2-2-phenylindole; MOI, multiplicity of infection; SD, standard deviation.
Pnl4 3 Deltaenv Gfp, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/immunocal+reagent/Sphaerellopsis+filum+(Bivona-Bernardi+%3A+Fries)+Sutton%2C+anamorph/pmc10848312-912-16-14
Average 94 stars, based on 1 article reviews
pnl4 3 deltaenv gfp - by Bioz Stars, 2026-09
94/100 stars
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packaging plasmids phef vsvg  (Addgene inc)
93
Addgene inc packaging plasmids phef vsvg
The effectiveness of Vpx incorporation into anti-HIV-1 gene therapy vectors. Macrophages were transduced with either Cal-1, PromA or their respective controls packaged with or without Vpx. At 14 dpt, the macrophages were infected with R5 BaL at MOI 0.2. After 7 days of infection, the cells were fixed and stained for <t>p17/p24</t> and counterstained with DAPI. The cells were imaged on the Cytell and analysed using IN Cell Investigator software. Using DAPI as the denominator the percentage of transduced cells was calculated for Cal-1 ( a ) and shPromA ( b ), the percentage of infected cells for Cal-1 ( c ) and shPromA ( e ), and the percentage of transduced and infected cells for Cal-1 ( d ) and shPromA ( f ). The nonparametric Friedman test was used for statistical analyses. P ≤ 0.05 (*), P ≤ 0.01 (**) and P ≤ 0.001 (***). Mean + SD were plotted; n = 3. One representative donor of three is displayed. DAPI, 4,6-diamidino-2-2-phenylindole; MOI, multiplicity of infection; SD, standard deviation.
Packaging Plasmids Phef Vsvg, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/immunocal+reagent/pHEF-VSVG+(Plasmid+%2322501)/10__1128_slash_jvi__01265___10-94-57-70
Average 93 stars, based on 1 article reviews
packaging plasmids phef vsvg - by Bioz Stars, 2026-09
93/100 stars
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packaging plasmid pspax2  (Addgene inc)
98
Addgene inc packaging plasmid pspax2
The effectiveness of Vpx incorporation into anti-HIV-1 gene therapy vectors. Macrophages were transduced with either Cal-1, PromA or their respective controls packaged with or without Vpx. At 14 dpt, the macrophages were infected with R5 BaL at MOI 0.2. After 7 days of infection, the cells were fixed and stained for <t>p17/p24</t> and counterstained with DAPI. The cells were imaged on the Cytell and analysed using IN Cell Investigator software. Using DAPI as the denominator the percentage of transduced cells was calculated for Cal-1 ( a ) and shPromA ( b ), the percentage of infected cells for Cal-1 ( c ) and shPromA ( e ), and the percentage of transduced and infected cells for Cal-1 ( d ) and shPromA ( f ). The nonparametric Friedman test was used for statistical analyses. P ≤ 0.05 (*), P ≤ 0.01 (**) and P ≤ 0.001 (***). Mean + SD were plotted; n = 3. One representative donor of three is displayed. DAPI, 4,6-diamidino-2-2-phenylindole; MOI, multiplicity of infection; SD, standard deviation.
Packaging Plasmid Pspax2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/immunocal+reagent/psPAX2+(Plasmid+%2312260)/pmc09927065-331-1-14
Average 98 stars, based on 1 article reviews
packaging plasmid pspax2 - by Bioz Stars, 2026-09
98/100 stars
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6 diamidino 2 phenylindole dapi  (Beyotime)
99
Beyotime 6 diamidino 2 phenylindole dapi
The effectiveness of Vpx incorporation into anti-HIV-1 gene therapy vectors. Macrophages were transduced with either Cal-1, PromA or their respective controls packaged with or without Vpx. At 14 dpt, the macrophages were infected with R5 BaL at MOI 0.2. After 7 days of infection, the cells were fixed and stained for <t>p17/p24</t> and counterstained with DAPI. The cells were imaged on the Cytell and analysed using IN Cell Investigator software. Using DAPI as the denominator the percentage of transduced cells was calculated for Cal-1 ( a ) and shPromA ( b ), the percentage of infected cells for Cal-1 ( c ) and shPromA ( e ), and the percentage of transduced and infected cells for Cal-1 ( d ) and shPromA ( f ). The nonparametric Friedman test was used for statistical analyses. P ≤ 0.05 (*), P ≤ 0.01 (**) and P ≤ 0.001 (***). Mean + SD were plotted; n = 3. One representative donor of three is displayed. DAPI, 4,6-diamidino-2-2-phenylindole; MOI, multiplicity of infection; SD, standard deviation.
6 Diamidino 2 Phenylindole Dapi, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/immunocal+reagent/DAPI/10__1016_slash_j__jia__2025__10__024-43-5-13
Average 99 stars, based on 1 article reviews
6 diamidino 2 phenylindole dapi - by Bioz Stars, 2026-09
99/100 stars
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resource source identifier alexa fluor 488 affinipure goat anti-human igg  (Jackson Immuno)
94
Jackson Immuno resource source identifier alexa fluor 488 affinipure goat anti-human igg
The effectiveness of Vpx incorporation into anti-HIV-1 gene therapy vectors. Macrophages were transduced with either Cal-1, PromA or their respective controls packaged with or without Vpx. At 14 dpt, the macrophages were infected with R5 BaL at MOI 0.2. After 7 days of infection, the cells were fixed and stained for <t>p17/p24</t> and counterstained with DAPI. The cells were imaged on the Cytell and analysed using IN Cell Investigator software. Using DAPI as the denominator the percentage of transduced cells was calculated for Cal-1 ( a ) and shPromA ( b ), the percentage of infected cells for Cal-1 ( c ) and shPromA ( e ), and the percentage of transduced and infected cells for Cal-1 ( d ) and shPromA ( f ). The nonparametric Friedman test was used for statistical analyses. P ≤ 0.05 (*), P ≤ 0.01 (**) and P ≤ 0.001 (***). Mean + SD were plotted; n = 3. One representative donor of three is displayed. DAPI, 4,6-diamidino-2-2-phenylindole; MOI, multiplicity of infection; SD, standard deviation.
Resource Source Identifier Alexa Fluor 488 Affinipure Goat Anti Human Igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/immunocal+reagent/Alexa+Fluor+488+AffiniPure+Goat+Anti-Human+IgG/pm37053069-574-2-16
Average 94 stars, based on 1 article reviews
resource source identifier alexa fluor 488 affinipure goat anti-human igg - by Bioz Stars, 2026-09
94/100 stars
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pe conjugated donkey anti mouse secondary reagent  (Jackson Immuno)
96
Jackson Immuno pe conjugated donkey anti mouse secondary reagent
The effectiveness of Vpx incorporation into anti-HIV-1 gene therapy vectors. Macrophages were transduced with either Cal-1, PromA or their respective controls packaged with or without Vpx. At 14 dpt, the macrophages were infected with R5 BaL at MOI 0.2. After 7 days of infection, the cells were fixed and stained for <t>p17/p24</t> and counterstained with DAPI. The cells were imaged on the Cytell and analysed using IN Cell Investigator software. Using DAPI as the denominator the percentage of transduced cells was calculated for Cal-1 ( a ) and shPromA ( b ), the percentage of infected cells for Cal-1 ( c ) and shPromA ( e ), and the percentage of transduced and infected cells for Cal-1 ( d ) and shPromA ( f ). The nonparametric Friedman test was used for statistical analyses. P ≤ 0.05 (*), P ≤ 0.01 (**) and P ≤ 0.001 (***). Mean + SD were plotted; n = 3. One representative donor of three is displayed. DAPI, 4,6-diamidino-2-2-phenylindole; MOI, multiplicity of infection; SD, standard deviation.
Pe Conjugated Donkey Anti Mouse Secondary Reagent, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/immunocal+reagent/Donkey+Anti-Mouse+IgG/pmc03826667-154-18-23
Average 96 stars, based on 1 article reviews
pe conjugated donkey anti mouse secondary reagent - by Bioz Stars, 2026-09
96/100 stars
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Image Search Results


The effectiveness of Vpx incorporation into anti-HIV-1 gene therapy vectors. Macrophages were transduced with either Cal-1, PromA or their respective controls packaged with or without Vpx. At 14 dpt, the macrophages were infected with R5 BaL at MOI 0.2. After 7 days of infection, the cells were fixed and stained for p17/p24 and counterstained with DAPI. The cells were imaged on the Cytell and analysed using IN Cell Investigator software. Using DAPI as the denominator the percentage of transduced cells was calculated for Cal-1 ( a ) and shPromA ( b ), the percentage of infected cells for Cal-1 ( c ) and shPromA ( e ), and the percentage of transduced and infected cells for Cal-1 ( d ) and shPromA ( f ). The nonparametric Friedman test was used for statistical analyses. P ≤ 0.05 (*), P ≤ 0.01 (**) and P ≤ 0.001 (***). Mean + SD were plotted; n = 3. One representative donor of three is displayed. DAPI, 4,6-diamidino-2-2-phenylindole; MOI, multiplicity of infection; SD, standard deviation.

Journal: Molecular Therapy. Methods & Clinical Development

Article Title: The feasibility of incorporating Vpx into lentiviral gene therapy vectors

doi: 10.1038/mtm.2016.66

Figure Lengend Snippet: The effectiveness of Vpx incorporation into anti-HIV-1 gene therapy vectors. Macrophages were transduced with either Cal-1, PromA or their respective controls packaged with or without Vpx. At 14 dpt, the macrophages were infected with R5 BaL at MOI 0.2. After 7 days of infection, the cells were fixed and stained for p17/p24 and counterstained with DAPI. The cells were imaged on the Cytell and analysed using IN Cell Investigator software. Using DAPI as the denominator the percentage of transduced cells was calculated for Cal-1 ( a ) and shPromA ( b ), the percentage of infected cells for Cal-1 ( c ) and shPromA ( e ), and the percentage of transduced and infected cells for Cal-1 ( d ) and shPromA ( f ). The nonparametric Friedman test was used for statistical analyses. P ≤ 0.05 (*), P ≤ 0.01 (**) and P ≤ 0.001 (***). Mean + SD were plotted; n = 3. One representative donor of three is displayed. DAPI, 4,6-diamidino-2-2-phenylindole; MOI, multiplicity of infection; SD, standard deviation.

Article Snippet: Macrophages were stained with the monoclonal antibodies p17 (ATCC; clone MH-SVM33C9) and p24 (Obtained from Bryan R Cullen through NIH AIDS Reagent Program, Division of AIDS, NIAID, NIH: pWT/BaL (Catalogue No. 11414 69 ).

Techniques: Transduction, Infection, Staining, Software, Standard Deviation