immunocal reagent Search Results


p24  (ATCC)
96
ATCC p24
The effectiveness of Vpx incorporation into anti-HIV-1 gene therapy vectors. Macrophages were transduced with either Cal-1, PromA or their respective controls packaged with or without Vpx. At 14 dpt, the macrophages were infected with R5 BaL at MOI 0.2. After 7 days of infection, the cells were fixed and stained for <t>p17/p24</t> and counterstained with DAPI. The cells were imaged on the Cytell and analysed using IN Cell Investigator software. Using DAPI as the denominator the percentage of transduced cells was calculated for Cal-1 ( a ) and shPromA ( b ), the percentage of infected cells for Cal-1 ( c ) and shPromA ( e ), and the percentage of transduced and infected cells for Cal-1 ( d ) and shPromA ( f ). The nonparametric Friedman test was used for statistical analyses. P ≤ 0.05 (*), P ≤ 0.01 (**) and P ≤ 0.001 (***). Mean + SD were plotted; n = 3. One representative donor of three is displayed. DAPI, 4,6-diamidino-2-2-phenylindole; MOI, multiplicity of infection; SD, standard deviation.
P24, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/p24/product/ATCC
Average 96 stars, based on 1 article reviews
p24 - by Bioz Stars, 2026-06
96/100 stars
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efavirenz  (Selleck Chemicals)
92
Selleck Chemicals efavirenz
The effectiveness of Vpx incorporation into anti-HIV-1 gene therapy vectors. Macrophages were transduced with either Cal-1, PromA or their respective controls packaged with or without Vpx. At 14 dpt, the macrophages were infected with R5 BaL at MOI 0.2. After 7 days of infection, the cells were fixed and stained for <t>p17/p24</t> and counterstained with DAPI. The cells were imaged on the Cytell and analysed using IN Cell Investigator software. Using DAPI as the denominator the percentage of transduced cells was calculated for Cal-1 ( a ) and shPromA ( b ), the percentage of infected cells for Cal-1 ( c ) and shPromA ( e ), and the percentage of transduced and infected cells for Cal-1 ( d ) and shPromA ( f ). The nonparametric Friedman test was used for statistical analyses. P ≤ 0.05 (*), P ≤ 0.01 (**) and P ≤ 0.001 (***). Mean + SD were plotted; n = 3. One representative donor of three is displayed. DAPI, 4,6-diamidino-2-2-phenylindole; MOI, multiplicity of infection; SD, standard deviation.
Efavirenz, supplied by Selleck Chemicals, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/efavirenz/product/Selleck Chemicals
Average 92 stars, based on 1 article reviews
efavirenz - by Bioz Stars, 2026-06
92/100 stars
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pe conjugated donkey anti mouse secondary reagent  (Jackson Immuno)
96
Jackson Immuno pe conjugated donkey anti mouse secondary reagent
The effectiveness of Vpx incorporation into anti-HIV-1 gene therapy vectors. Macrophages were transduced with either Cal-1, PromA or their respective controls packaged with or without Vpx. At 14 dpt, the macrophages were infected with R5 BaL at MOI 0.2. After 7 days of infection, the cells were fixed and stained for <t>p17/p24</t> and counterstained with DAPI. The cells were imaged on the Cytell and analysed using IN Cell Investigator software. Using DAPI as the denominator the percentage of transduced cells was calculated for Cal-1 ( a ) and shPromA ( b ), the percentage of infected cells for Cal-1 ( c ) and shPromA ( e ), and the percentage of transduced and infected cells for Cal-1 ( d ) and shPromA ( f ). The nonparametric Friedman test was used for statistical analyses. P ≤ 0.05 (*), P ≤ 0.01 (**) and P ≤ 0.001 (***). Mean + SD were plotted; n = 3. One representative donor of three is displayed. DAPI, 4,6-diamidino-2-2-phenylindole; MOI, multiplicity of infection; SD, standard deviation.
Pe Conjugated Donkey Anti Mouse Secondary Reagent, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pe conjugated donkey anti mouse secondary reagent/product/Jackson Immuno
Average 96 stars, based on 1 article reviews
pe conjugated donkey anti mouse secondary reagent - by Bioz Stars, 2026-06
96/100 stars
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intraprep permeabilization fixation reagent kit  (Beckman Coulter)
96
Beckman Coulter intraprep permeabilization fixation reagent kit
The effectiveness of Vpx incorporation into anti-HIV-1 gene therapy vectors. Macrophages were transduced with either Cal-1, PromA or their respective controls packaged with or without Vpx. At 14 dpt, the macrophages were infected with R5 BaL at MOI 0.2. After 7 days of infection, the cells were fixed and stained for <t>p17/p24</t> and counterstained with DAPI. The cells were imaged on the Cytell and analysed using IN Cell Investigator software. Using DAPI as the denominator the percentage of transduced cells was calculated for Cal-1 ( a ) and shPromA ( b ), the percentage of infected cells for Cal-1 ( c ) and shPromA ( e ), and the percentage of transduced and infected cells for Cal-1 ( d ) and shPromA ( f ). The nonparametric Friedman test was used for statistical analyses. P ≤ 0.05 (*), P ≤ 0.01 (**) and P ≤ 0.001 (***). Mean + SD were plotted; n = 3. One representative donor of three is displayed. DAPI, 4,6-diamidino-2-2-phenylindole; MOI, multiplicity of infection; SD, standard deviation.
Intraprep Permeabilization Fixation Reagent Kit, supplied by Beckman Coulter, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/intraprep permeabilization fixation reagent kit/product/Beckman Coulter
Average 96 stars, based on 1 article reviews
intraprep permeabilization fixation reagent kit - by Bioz Stars, 2026-06
96/100 stars
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cas block agent  (Jackson Immuno)
91
Jackson Immuno cas block agent
The effectiveness of Vpx incorporation into anti-HIV-1 gene therapy vectors. Macrophages were transduced with either Cal-1, PromA or their respective controls packaged with or without Vpx. At 14 dpt, the macrophages were infected with R5 BaL at MOI 0.2. After 7 days of infection, the cells were fixed and stained for <t>p17/p24</t> and counterstained with DAPI. The cells were imaged on the Cytell and analysed using IN Cell Investigator software. Using DAPI as the denominator the percentage of transduced cells was calculated for Cal-1 ( a ) and shPromA ( b ), the percentage of infected cells for Cal-1 ( c ) and shPromA ( e ), and the percentage of transduced and infected cells for Cal-1 ( d ) and shPromA ( f ). The nonparametric Friedman test was used for statistical analyses. P ≤ 0.05 (*), P ≤ 0.01 (**) and P ≤ 0.001 (***). Mean + SD were plotted; n = 3. One representative donor of three is displayed. DAPI, 4,6-diamidino-2-2-phenylindole; MOI, multiplicity of infection; SD, standard deviation.
Cas Block Agent, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cas block agent/product/Jackson Immuno
Average 91 stars, based on 1 article reviews
cas block agent - by Bioz Stars, 2026-06
91/100 stars
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atp chemiluminescence assay kit  (Elabscience Biotechnology)
95
Elabscience Biotechnology atp chemiluminescence assay kit
The effectiveness of Vpx incorporation into anti-HIV-1 gene therapy vectors. Macrophages were transduced with either Cal-1, PromA or their respective controls packaged with or without Vpx. At 14 dpt, the macrophages were infected with R5 BaL at MOI 0.2. After 7 days of infection, the cells were fixed and stained for <t>p17/p24</t> and counterstained with DAPI. The cells were imaged on the Cytell and analysed using IN Cell Investigator software. Using DAPI as the denominator the percentage of transduced cells was calculated for Cal-1 ( a ) and shPromA ( b ), the percentage of infected cells for Cal-1 ( c ) and shPromA ( e ), and the percentage of transduced and infected cells for Cal-1 ( d ) and shPromA ( f ). The nonparametric Friedman test was used for statistical analyses. P ≤ 0.05 (*), P ≤ 0.01 (**) and P ≤ 0.001 (***). Mean + SD were plotted; n = 3. One representative donor of three is displayed. DAPI, 4,6-diamidino-2-2-phenylindole; MOI, multiplicity of infection; SD, standard deviation.
Atp Chemiluminescence Assay Kit, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/atp chemiluminescence assay kit/product/Elabscience Biotechnology
Average 95 stars, based on 1 article reviews
atp chemiluminescence assay kit - by Bioz Stars, 2026-06
95/100 stars
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peroxidase affinipure goat anti mouse igg  (Jackson Immuno)
96
Jackson Immuno peroxidase affinipure goat anti mouse igg
The effectiveness of Vpx incorporation into anti-HIV-1 gene therapy vectors. Macrophages were transduced with either Cal-1, PromA or their respective controls packaged with or without Vpx. At 14 dpt, the macrophages were infected with R5 BaL at MOI 0.2. After 7 days of infection, the cells were fixed and stained for <t>p17/p24</t> and counterstained with DAPI. The cells were imaged on the Cytell and analysed using IN Cell Investigator software. Using DAPI as the denominator the percentage of transduced cells was calculated for Cal-1 ( a ) and shPromA ( b ), the percentage of infected cells for Cal-1 ( c ) and shPromA ( e ), and the percentage of transduced and infected cells for Cal-1 ( d ) and shPromA ( f ). The nonparametric Friedman test was used for statistical analyses. P ≤ 0.05 (*), P ≤ 0.01 (**) and P ≤ 0.001 (***). Mean + SD were plotted; n = 3. One representative donor of three is displayed. DAPI, 4,6-diamidino-2-2-phenylindole; MOI, multiplicity of infection; SD, standard deviation.
Peroxidase Affinipure Goat Anti Mouse Igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/peroxidase affinipure goat anti mouse igg/product/Jackson Immuno
Average 96 stars, based on 1 article reviews
peroxidase affinipure goat anti mouse igg - by Bioz Stars, 2026-06
96/100 stars
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fitc conjugated anti rabbit  (Jackson Immuno)
94
Jackson Immuno fitc conjugated anti rabbit
The effectiveness of Vpx incorporation into anti-HIV-1 gene therapy vectors. Macrophages were transduced with either Cal-1, PromA or their respective controls packaged with or without Vpx. At 14 dpt, the macrophages were infected with R5 BaL at MOI 0.2. After 7 days of infection, the cells were fixed and stained for <t>p17/p24</t> and counterstained with DAPI. The cells were imaged on the Cytell and analysed using IN Cell Investigator software. Using DAPI as the denominator the percentage of transduced cells was calculated for Cal-1 ( a ) and shPromA ( b ), the percentage of infected cells for Cal-1 ( c ) and shPromA ( e ), and the percentage of transduced and infected cells for Cal-1 ( d ) and shPromA ( f ). The nonparametric Friedman test was used for statistical analyses. P ≤ 0.05 (*), P ≤ 0.01 (**) and P ≤ 0.001 (***). Mean + SD were plotted; n = 3. One representative donor of three is displayed. DAPI, 4,6-diamidino-2-2-phenylindole; MOI, multiplicity of infection; SD, standard deviation.
Fitc Conjugated Anti Rabbit, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fitc conjugated anti rabbit/product/Jackson Immuno
Average 94 stars, based on 1 article reviews
fitc conjugated anti rabbit - by Bioz Stars, 2026-06
94/100 stars
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mouse anti rat igg fitc  (Jackson Immuno)
93
Jackson Immuno mouse anti rat igg fitc
The effectiveness of Vpx incorporation into anti-HIV-1 gene therapy vectors. Macrophages were transduced with either Cal-1, PromA or their respective controls packaged with or without Vpx. At 14 dpt, the macrophages were infected with R5 BaL at MOI 0.2. After 7 days of infection, the cells were fixed and stained for <t>p17/p24</t> and counterstained with DAPI. The cells were imaged on the Cytell and analysed using IN Cell Investigator software. Using DAPI as the denominator the percentage of transduced cells was calculated for Cal-1 ( a ) and shPromA ( b ), the percentage of infected cells for Cal-1 ( c ) and shPromA ( e ), and the percentage of transduced and infected cells for Cal-1 ( d ) and shPromA ( f ). The nonparametric Friedman test was used for statistical analyses. P ≤ 0.05 (*), P ≤ 0.01 (**) and P ≤ 0.001 (***). Mean + SD were plotted; n = 3. One representative donor of three is displayed. DAPI, 4,6-diamidino-2-2-phenylindole; MOI, multiplicity of infection; SD, standard deviation.
Mouse Anti Rat Igg Fitc, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse anti rat igg fitc/product/Jackson Immuno
Average 93 stars, based on 1 article reviews
mouse anti rat igg fitc - by Bioz Stars, 2026-06
93/100 stars
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antibody reagents  (Jackson Immuno)
96
Jackson Immuno antibody reagents
The effectiveness of Vpx incorporation into anti-HIV-1 gene therapy vectors. Macrophages were transduced with either Cal-1, PromA or their respective controls packaged with or without Vpx. At 14 dpt, the macrophages were infected with R5 BaL at MOI 0.2. After 7 days of infection, the cells were fixed and stained for <t>p17/p24</t> and counterstained with DAPI. The cells were imaged on the Cytell and analysed using IN Cell Investigator software. Using DAPI as the denominator the percentage of transduced cells was calculated for Cal-1 ( a ) and shPromA ( b ), the percentage of infected cells for Cal-1 ( c ) and shPromA ( e ), and the percentage of transduced and infected cells for Cal-1 ( d ) and shPromA ( f ). The nonparametric Friedman test was used for statistical analyses. P ≤ 0.05 (*), P ≤ 0.01 (**) and P ≤ 0.001 (***). Mean + SD were plotted; n = 3. One representative donor of three is displayed. DAPI, 4,6-diamidino-2-2-phenylindole; MOI, multiplicity of infection; SD, standard deviation.
Antibody Reagents, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/antibody reagents/product/Jackson Immuno
Average 96 stars, based on 1 article reviews
antibody reagents - by Bioz Stars, 2026-06
96/100 stars
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supt1 cells  (ATCC)
97
ATCC supt1 cells
The effectiveness of Vpx incorporation into anti-HIV-1 gene therapy vectors. Macrophages were transduced with either Cal-1, PromA or their respective controls packaged with or without Vpx. At 14 dpt, the macrophages were infected with R5 BaL at MOI 0.2. After 7 days of infection, the cells were fixed and stained for <t>p17/p24</t> and counterstained with DAPI. The cells were imaged on the Cytell and analysed using IN Cell Investigator software. Using DAPI as the denominator the percentage of transduced cells was calculated for Cal-1 ( a ) and shPromA ( b ), the percentage of infected cells for Cal-1 ( c ) and shPromA ( e ), and the percentage of transduced and infected cells for Cal-1 ( d ) and shPromA ( f ). The nonparametric Friedman test was used for statistical analyses. P ≤ 0.05 (*), P ≤ 0.01 (**) and P ≤ 0.001 (***). Mean + SD were plotted; n = 3. One representative donor of three is displayed. DAPI, 4,6-diamidino-2-2-phenylindole; MOI, multiplicity of infection; SD, standard deviation.
Supt1 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/supt1 cells/product/ATCC
Average 97 stars, based on 1 article reviews
supt1 cells - by Bioz Stars, 2026-06
97/100 stars
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protein g plus agarose immuno precipitation reagent  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology protein g plus agarose immuno precipitation reagent
The effectiveness of Vpx incorporation into anti-HIV-1 gene therapy vectors. Macrophages were transduced with either Cal-1, PromA or their respective controls packaged with or without Vpx. At 14 dpt, the macrophages were infected with R5 BaL at MOI 0.2. After 7 days of infection, the cells were fixed and stained for <t>p17/p24</t> and counterstained with DAPI. The cells were imaged on the Cytell and analysed using IN Cell Investigator software. Using DAPI as the denominator the percentage of transduced cells was calculated for Cal-1 ( a ) and shPromA ( b ), the percentage of infected cells for Cal-1 ( c ) and shPromA ( e ), and the percentage of transduced and infected cells for Cal-1 ( d ) and shPromA ( f ). The nonparametric Friedman test was used for statistical analyses. P ≤ 0.05 (*), P ≤ 0.01 (**) and P ≤ 0.001 (***). Mean + SD were plotted; n = 3. One representative donor of three is displayed. DAPI, 4,6-diamidino-2-2-phenylindole; MOI, multiplicity of infection; SD, standard deviation.
Protein G Plus Agarose Immuno Precipitation Reagent, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/protein g plus agarose immuno precipitation reagent/product/Santa Cruz Biotechnology
Average 96 stars, based on 1 article reviews
protein g plus agarose immuno precipitation reagent - by Bioz Stars, 2026-06
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Image Search Results


The effectiveness of Vpx incorporation into anti-HIV-1 gene therapy vectors. Macrophages were transduced with either Cal-1, PromA or their respective controls packaged with or without Vpx. At 14 dpt, the macrophages were infected with R5 BaL at MOI 0.2. After 7 days of infection, the cells were fixed and stained for p17/p24 and counterstained with DAPI. The cells were imaged on the Cytell and analysed using IN Cell Investigator software. Using DAPI as the denominator the percentage of transduced cells was calculated for Cal-1 ( a ) and shPromA ( b ), the percentage of infected cells for Cal-1 ( c ) and shPromA ( e ), and the percentage of transduced and infected cells for Cal-1 ( d ) and shPromA ( f ). The nonparametric Friedman test was used for statistical analyses. P ≤ 0.05 (*), P ≤ 0.01 (**) and P ≤ 0.001 (***). Mean + SD were plotted; n = 3. One representative donor of three is displayed. DAPI, 4,6-diamidino-2-2-phenylindole; MOI, multiplicity of infection; SD, standard deviation.

Journal: Molecular Therapy. Methods & Clinical Development

Article Title: The feasibility of incorporating Vpx into lentiviral gene therapy vectors

doi: 10.1038/mtm.2016.66

Figure Lengend Snippet: The effectiveness of Vpx incorporation into anti-HIV-1 gene therapy vectors. Macrophages were transduced with either Cal-1, PromA or their respective controls packaged with or without Vpx. At 14 dpt, the macrophages were infected with R5 BaL at MOI 0.2. After 7 days of infection, the cells were fixed and stained for p17/p24 and counterstained with DAPI. The cells were imaged on the Cytell and analysed using IN Cell Investigator software. Using DAPI as the denominator the percentage of transduced cells was calculated for Cal-1 ( a ) and shPromA ( b ), the percentage of infected cells for Cal-1 ( c ) and shPromA ( e ), and the percentage of transduced and infected cells for Cal-1 ( d ) and shPromA ( f ). The nonparametric Friedman test was used for statistical analyses. P ≤ 0.05 (*), P ≤ 0.01 (**) and P ≤ 0.001 (***). Mean + SD were plotted; n = 3. One representative donor of three is displayed. DAPI, 4,6-diamidino-2-2-phenylindole; MOI, multiplicity of infection; SD, standard deviation.

Article Snippet: Macrophages were stained with the monoclonal antibodies p17 (ATCC; clone MH-SVM33C9) and p24 (Obtained from Bryan R Cullen through NIH AIDS Reagent Program, Division of AIDS, NIAID, NIH: pWT/BaL (Catalogue No. 11414 69 ).

Techniques: Transduction, Infection, Staining, Software, Standard Deviation